In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity

Abstract As the COVID-19 infection continues to ravage world, advent of an efficient as well economization existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help management pandemic. This study demonstrated innovative rapid corroboration test on multiplex PCR. An assessment optimal PCR conditions simultaneously amplify SARS-CoV-2 genes E, S RdRp has been made by fast-conventional HRM coupled real-time using same sets primers. All variables practical value were studied amplifying known target-sequences from ten-fold dilutions archived positive samples disease. The multiplexing with newly designed primers have shown amplification target region SARS-CoV-2. A distinct was observed 37 min thermal cycler while it took 96 real time SYBR green over wide range template concentrations. Our findings revealed decent concordance other commercially available kits. fast approach offers sensitive cost-effective manner apart added advantage primer compatibility for use conventional highly reproducible novel propel extended applicability developing sustainable commercial product besides providing relief resource limited setting.